pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

LOCUS       pGreenIIM DR5v2-ntdTomato/DR5-n3GFP                9680 bp ds-DNA     circular SYN 01-OCT-2019
DEFINITION  Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) 
            promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D
  TITLE     Reporters for sensitive and quantitative measurement of auxin 
            response.
  JOURNAL   Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub 
            2015 Feb 2.
  PUBMED    25643149
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(607..635)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1230..1250
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1634..1654)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1634..1652)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1673..1689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1673..1689)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1686..1708)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1697..1713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1721..1751)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1766..1787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1904..1921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2026..2050
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2141..2729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2221..2240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2900..3715)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3623..3642
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3707..3726
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4006..4441
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    4568..4590
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    4598..4774
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5170..5733)
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDRPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     promoter        complement(5766..6111)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5803..5823)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     6314..6336
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6328..6345
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6329..6345
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6355..6374
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6355..6373
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        6602..6647
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    6658..6713
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6760..6780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6781..8211
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     promoter        8637..8682
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    8693..8748
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             8770..8790
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8791..9507
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             9514..553
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
       61 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      121 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      181 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      241 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      301 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      361 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      421 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
      481 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      541 gctgtacaag taaagatcca aggagatata accatgagta gtaaaggaga agaacttttc
      601 actggagttg tcccaattct tgttgaatta gatggtgatg gtaatgggca caaattttct
      661 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      721 actactggaa aactacctgt tccatggcca acacttgtca ctacgttaac ttatggagta
      781 caatgctttt caagataccc agatcatatg aagcggcacg acttcttcaa gagcgccatg
      841 cctgagggat acgtgcagga gaggaccatc ttcttcaagg acgacgggaa ctacaagaca
      901 cgtgctgaag tcaagtttga gggagacacc ctcgtcaaca ggatcgagct taagggaatc
      961 gatttcaagg aggacggaaa catcctcggc cacaagttgg aatacaacta caactcccac
     1021 aacgtataca tcatggcaga caaacaaaag aatggaatca aagtcaactt caaaattaga
     1081 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1141 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1201 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1261 attacacatg gcatggatga atacaaatct tacgttactg ttccaaacag aactagaact
     1321 tacatcaagt aatctagatc cgatccccga tcgttcaaac atttggcaat aaagtttctt
     1381 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     1441 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     1501 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     1561 ggataaatta tcgcgcgcgg tgtcatctat gtctagagcg gccgccaccg cggtggagct
     1621 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     1681 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1741 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1801 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1861 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1921 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1981 tatccacaga atcaggggat aacgcaggaa agaacatgaa ggccttgaca ggatatattg
     2041 gcgggtaaac taagtcgctg tatgtgtttg tttgagatct catgtgagca aaaggccagc
     2101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaga agagttggta
     2641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2881 tgtaacattg gtctagtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     2941 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     3001 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     3061 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     3121 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     3181 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     3241 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     3301 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     3361 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag
     3421 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     3481 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     3541 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg
     3601 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     3661 tgttggaatt taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacac
     3721 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     3781 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa
     3841 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
     3901 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
     3961 gctccctcac tttctggctg gatgatgggg cgattcaggc gatccccatc caacagcccg
     4021 ccgtcgagcg ggctttttta tccccggaag cctgtggata gagggtagtt atccacgtga
     4081 aaccgctaat gccccgcaaa gccttgattc acggggcttt ccggcccgct ccaaaaacta
     4141 tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa tcggagtacg tgaaatcgct
     4201 aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga gaacgctaat agccctttca
     4261 gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt tacagcaagt agtatgttca
     4321 attagctttt caattatgaa tatatatatc aattattggt cgcccttggc ttgtggacaa
     4381 tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag cggttgccca ccgtcgagcg
     4441 cctttgccca caacccggcg gccggccgca acagatcgtt ttataaattt ttttttttga
     4501 aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct ggatttccga tccccggaat
     4561 tagatcttgg caggatatat tgtggtgtaa cgttatcgat ctggatttta gtactggatt
     4621 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact
     4681 aagggtttct tatatgctca acacatgagc gaaaccctat aagaacccta atttccctta
     4741 tcgggaaact actcacacat tatttatgga gaaaatagag agagatagat ttgtagagag
     4801 agactggtga tttcagcgta ccgaattaat tcgagctcgg tacccctgga ttttggtttt
     4861 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
     4921 cttatatgct caacacatga gcgaaaccct ataagaaccc taattccctt atctgggaac
     4981 tactcacaca ttattataga gagagataga tttgtagaga gagactggtg atttcagcgg
     5041 gggattcagc gccgacggga cgtaaacaaa ggacgtcccg cgaaggatct aaagccagca
     5101 aaagtcccat ggtcttataa aaatgcatag ctttaggagg ggagcagaga acttgaaagc
     5161 atcttcctgt tagtctttct tctcgtagac ttcaaactta tacttgatgc ctttttcctc
     5221 ctggacctca gagaggacgc ctgggtattc tgggagaagt ttatatttcc ccaaatcaat
     5281 ttctgggaaa aacgtgtcac tttcaaattc ctgcatgatc cttgtcacaa agagtctgag
     5341 gtggcctggt tgattcatgg cttcctggta aacagaactg cctccgacta tccaaaccat
     5401 gtctacttta cttgccaatt ccggttgttc aataagtctt aaggcatcat ccaaactttt
     5461 ggcaagaaaa tgagctcctc gtggtggttc tttgagttct ctactgagaa ctatattaat
     5521 tctgtccttt aaaggtcgat tcttctcagg aatggagaac caggttttcc tacccataat
     5581 caccagattc tgtttacctt ccactgaaga ggttgtggtc attctttgga agtacttgaa
     5641 ctcgttcctg agcggaggcc agggtcggtc tccgttcttg ccaatcccca tattttggga
     5701 cacggcgacg atgcagttca atggtcgaac catgatggca aattctaggg gtacccagct
     5761 tgggctgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga
     5821 tagtgggatt gtgcgtcatc ccttacgtca gtggagatgt cacatcaatc cacttgcttt
     5881 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
     5941 ttgggaccac tgtcggcaga ggcatcttga atgatagcct ttcctttatc gcaatgatgg
     6001 catttgtagg agccaccttc cttttctact gtcctttcga tgaagtgaca gatagctggg
     6061 caatggaatc cgaggaggtt tcccgaaatt accctttgtt gaaaagtctc aatagccctt
     6121 tggtcttctg agactgtatc tttgacattt ttggagtagg ggtacgataa cattaacgct
     6181 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     6241 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     6301 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     6361 gactcactat agggcgaatt gggtaccggg cccgtatggt taattaagac acaggcgcgc
     6421 ctagttggaa taggatttcg aattcccgac aaaaggccga caaaaggccg acaaaaggcc
     6481 gacaaaaggc cgacaaaagg ccgacaaaag gccgacaaaa ggccgacaaa aggccgacaa
     6541 aaggccgaca aaagggggca ggcctcgata agcttgatat cgaattaatt cctgcagccc
     6601 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggtat ttttacaaca
     6661 attaccaaca acaacaaaca acaaacaaca ttacaattac tatttacaat tacaattaca
     6721 ggggatccga aatccaactc catactgaag cttatggctc caaagaagaa gagaaaggtc
     6781 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     6841 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     6901 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     6961 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     7021 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     7081 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     7141 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     7201 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     7261 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     7321 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     7381 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     7441 cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc
     7501 gcctcctccg aggacaacaa catggccgtc atcaaagagt tcatgcgctt caaggtgcgc
     7561 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     7621 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     7681 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     7741 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     7801 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     7861 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     7921 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     7981 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     8041 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     8101 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     8161 gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaagta gctcgagaga
     8221 tccgatcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     8281 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     8341 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     8401 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     8461 ggtgtcatct atgtgaattc gagacaaaag ggagacaaaa gggagacaaa agggagacaa
     8521 aagggagaca aaagggagac aaaagggaga caaaagggag acaaaaggga gacaaaaggg
     8581 agacaaaagg gggcaggcct cgataagctt gatatcgaat taattcctgc agccccgcaa
     8641 gacccttcct ctatataagg aagttcattt catttggaga ggtattttta caacaattac
     8701 caacaacaac aaacaacaaa caacattaca attactattt acaattacaa ttacagggga
     8761 tccatggctc caaagaagaa gagaaaggtc atggtgagca agggcgagga gctgttcacc
     8821 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     8881 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     8941 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     9001 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     9061 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     9121 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     9181 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     9241 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     9301 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     9361 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     9421 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     9481 actctcggca tggacgagct gtacaagaga tccatggtga gcaagggcga ggagctgttc
     9541 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     9601 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     9661 accaccggca agctgcccgt
//